IKEBUKURO Kazunori

Professional Career

1993.04 - 1996.03- 東京大学　先端科学技術研究センター　助手
1996.04 - 2001.03- 東京大学　先端科学技術研究センター　講師
2001.04 - present : 東京農工大学　工学部

Academic Degrees

1992 Mc.S. in Engineering, The University of Tokyo
1993 Ph.D. in Engineering, The University of Tokyo

Research Interests

Biosensor, aptamer, Evolutionary molecular engineering, DNA/SNP sensor

Publications

• Hiraka, K., Yoshida, H., Tsugawa, W., Asano, R., LaBelle, J., Ikebukuro, K., Sode, K. Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product-inhibition mechanism and oxygen gatekeeper. Protein Science (Protein Sci.). 31:e4434, 2022
• Tu, A.T.T., Hoshi, K., Ma, Y., Oyama, T., Suzuki, S., Tsukakoshi, K., Nagasawa, K., Ikebukuro, K. & Yamazaki, T., Effects of G-Quadruplex Ligands on the Topology, Stability, and Immunostimulatory Properties of G-Quadruplex-Based CpG Oligodeoxynucleotides. ACS Chem Biol 17, 1703-1713 (2022)
• Khanwalker M, Fujita R, Lee J, Wilson E, Ito K, Asano R, Ikebukuro K, LaBelle J, Sode K, Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement, Biosens, Bioelectron., 200, 113901, doi: 10.1016/j.bios.2021.113901, 2022
• Nagata M, Lee J, Henley S, Ikebukuro K, Sode K, An Amine-Reactive Phenazine Ethosulfate (arPES)-A Novel Redox Probe for Electrochemical Aptamer-Based Sensor, Sensors (Basel), 22(5), 1760, 2022.
• Takamatsu S, Lee I, Lee J, Asano R, Tsugawa W, Ikebukuro K, Dick JE, Sode K, Transient potentiometry based d-serine sensor using engineered d-amino acid oxidase showing quasi-direct electron transfer property, Biosens. Bioelectron., 200, 113927, doi: 10.1016/j.bios.2021.113927, 2022
• Kobayashi S, Atsumi S, Ikebukuro K, Sode K, Asano R, Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria, Microb. Cell Fact., 21(1), 7, doi: 10.1186/s12934-021-01732-x., 2022
• Safitri FA, Tu ATT, Hoshi K, Shobo M, Zhao D, Witarto AB, Sumarsono SH, Giri-Rachman EA, Tsukakoshi K, Ikebukuro K, Yamazaki T, Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparallel Guanine-Quadruplex Structural Scaffold, Biomolecules, 11(11), 1617, doi: 10.3390/biom11111617, 2021
• Miura D, Kimura H, Tsugawa W, Ikebukuro K, Sode K, Asano R, Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody-enzyme complex, Talanta, 234, 122638, doi: 10.1016/j.talanta.2021.122638., 2021.
• Ariyanti D, Ikebukuro K, Sode K., Artificial complementary chromatic acclimation gene expression system in Escherichia coli, Microb Cell Fact, 20(1), 128, doi: 10.1186/s12934-021-01621-3., 2021.
• Tsukakoshi K, Yamagishi Y, Kanazashi M, Nakama K, Oshikawa D, Savory N, Matsugami A, Hayashi F, Lee J, Saito T, Sode K, Khunathai K, Kuno H, Ikebukuro K, G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol, Nucleic Acids Res. 21, 49(11), 6069-6081. doi: 10.1093/nar/gkab388., 2021
• Kimura H, Miura D, Tsugawa W, Ikebukuro K, Sode K, Asano R., Rapid and homogeneous electrochemical detection by fabricating a high affinity bispecific antibody-enzyme complex using two Catcher/Tag systems, Biosensors and Bioelectronics, 175, 112885. DOI: 10.1016/j.bios.2020.112885
• Hatada M, Saito S, Yonehara S, Tsugawa W, Asano R, Ikebukuro K, Sode K., Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase, Biosensors and Bioelectronics, 177, 112984, 2021, DOI: 10.1016/j.bios.2021.112984.
• Hiraka K, Tsugawa W, Asano R, Yokus MA, Ikebukuro K, Daniele MA, Sode K., Rational design of direct electron transfer type l-lactate dehydrogenase for the development of multiplexed biosensor, Biosensors and Bioelectronics, 176, 112933. DOI: 10.1016/j.bios.2020.112933.
• Ito K, Okuda-Shimazaki J, Kojima K, Mori K, Tsugawa W, Asano R, Ikebukuro K, Sode K, Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors, Biosensors and Bioelectronics, 176, 112911. DOI: 10.1016/j.bios.2020.112911, 2021
• Shono, C., Ariyanti, 2D., Abe, K., Sakai, Y., Sakamoto, I., Tsukakoshi, K., Sode, K., Ikebukuro, K., A green light-regulated T7 RNA polymerase 1 gene expression system 2 for cyanobacteria, Marine Biotechnology(Mar. Biotechnol.), DOI: 10.1007/s10126-020-09997-w
• Lee, J., Tatsumi, A., Tsukakoshi, K., Wilson, E.D., Abe, K., Sode, K., Ikebukuro, K., Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF, Sensors (Basel), 20(14), 3878, 2020
• Fitriana, M., Loew, N., Witarto, A.B., Ikebukuro, K., Sode, K., Tsugawa, W., Employment of 1-Methoxy-5-Ethyl Phenazinium Ethyl Sulfate as a Stable Electron Mediator in Flavin Oxidoreductases-Based Sensors, Sensors (Basel), 20(10), 2825, 2020
• Hiraka, K., Kojima, K., Tsugawa, W., Asano, R., Ikebukuro, K., Sode, K., Rational engineering of Aerococcus viridans l-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor. Biosensors and Bioelectronics (Biosens. Bioelectron.), 151, 111974, 2020.
• Lee, I., Loew, N., Tsugawa, W., Ikebukuro, K., Sode, K. Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring. Biosensors and Bioelectronics (Bios. Bioelectron. 124-125: 216-223, 2019
• Tsukakoshi, K., Yoshida, W., Kobayashi, M., Kobayashi, N., Kim, J., Kaku, T., Iguchi, T., Nagasawa, K., Asano, R., Ikebukuro, K., Sode, K. Esterification of PQQ Enhances Blood-Brain Barrier Permeability and Inhibitory Activity against Amyloidogenic Protein Fibril Formation. ACS Chemical Neuroscience (ACS Chem Neurosci). doi: 10.1021/acschemneuro.8b00355, 9:12: 2898-2903
• Hoshi, K., Yamazaki, T., Yoshikawa, C., Tsugawa, W., Ikebukuro, K., Sode, K. Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production. International journal of Nanomedicine (Int. J Nanomedicine). 13:4461-4472, 2018