| Date | 2026.7.24 (16：00 - 17：30) |
|---|---|
| Venue |
Meeting ID:837 5426 0027 Passcode:300047 |
| Speaker | Dr. Clotilde Policar Ecole Normale Supérieure, - PSL university (France) |
| Title | "Metal complexes in biology: metal-based antioxidants as enzymes mimics and metal-CO as probes" ＜Abstract＞ After a general introduction on bioinorganic chemistry, some of its history and current challenges [1], the talk will focus on the design of metal-based catalytic antioxidants and the development of Re(CO) compounds as metal-based probes. Metal complexes are increasingly used for biological applications, as metalloprobes or metallodrugs [2]. Cell penetration, in-cell distribution and speciation are important to their bio-activity. Because of the nature of biological media (high viscosity, molecular overcrowding, compartmented environment, or high content in Lewis bases and metal ions), their speciation and activity can be impacted by the cellular surroundings and a compound intrinsically active (out of any cellular context) may not keep its activity in cells. It is therefore key to study metal complexes meant for therapeutic purposes directly in biological environments and correlate bioactivity with information on intracellular distribution and speciation [1,3]. The conference will describe how metal-based antioxidants, developed as mimics of superoxide dismutase, can be studied directly in cells: evaluation of the bioactivity [4–6], imaging [4,7], analyses of their speciation [6,8], and evaluation of the cellular redox state by a redoxomic approach [9]. Their usage of the antioxidants as anti-inflammatory agents in a cellular model in link with inflammatory bowel diseases using bacteria for delivery in cells and in vivo [10]. In a second part, we will focus on application of metal-based probes and their use for multimodal imaging.[11–13] More specifically, probes consisting of a central metal-CO core, called SCoMPIs (for single core multimodal probes for imaging), can be mapped using unconventional imaging techniques such as IR and X-fluorescence imagings. Several examples, including a SOD mimic conjugated with a Re(CO)3-based probe,[7] with imaging used to explore the integrity of the complex[8] in cells, and the design of Re(CO)3-based organelle trackers,[14,15] that can be used for X-fluorescence mapping. [1] C. Policar, “Bioinorganic chemistry: where from and where to?” J.B.I.C. 2025, Accepted. [2] N. J. Farrer, P. J. Sadler in Bioinorganic Medicinal Chemistry (Ed.: E. Alession), Wiley-VCH, Weinheim, Germany, 2011, pp. 1–47. [3] C. Policar, C. Rampon, A. Balfourier, M. Volovitch, S. Vriz, H. C. Bertrand, N. Delsuc, “Inorganic chemical biology and metal complexes in cells: from the design of cellular models to evaluate antioxidant activity to the characterization of metal complexes in cells” Comptes Rendus. Chimie 2025, 25, 397–420. [4] E. Mathieu, A.-S. Bernard, N. Delsuc, E. Quévrain, G. Gazzah, B. Lai, F. Chain, P. Langella, M. Bachelet, J. Masliah, P. Seksik, C. Policar, “A Cell-Penetrant Manganese Superoxide Dismutase (MnSOD) Mimic Is Able To Complement MnSOD and Exerts an Antiinflammatory Effect on Cellular and Animal Models of Inflammatory Bowel Diseases” Inorg. Chem. 2017, 56, 2545–2555. [5] A. Vincent, M. Thauvin, E. Quévrain, E. Mathieu, S. Layani, P. Seksik, I. Batinic-Haberle, S. Vriz, C. Policar, N. Delsuc, “Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models” J. Inorg. Biochem. 2021, 219, 111431. [6] G. Schanne, M. Zoumpoulaki, G. Gazzah, A. Vincent, H. Preud’homme, R. Lobinski, S. Demignot, P. Seksik, N. Delsuc, C. Policar, “Inertness of Superoxide Dismutase Mimics Mn(II) Complexes Based on an Open-Chain Ligand, Bioactivity, and Detection in Intestinal Epithelial Cells” Oxid. Med. Cell Longev. 2022, 2022, Article ID 3858122. [7] E. Mathieu, A.-S. Bernard, E. Quévrain, M. Zoumpoulaki, S. Iriart, C. Lung-Soong, B. Lai, K. Medjoubi, L. Henry, S. Nagarajan, F. Poyer, A. Scheitler, I. Ivanović-Burmazović, S. Marco, A. Somogyi, P. Seksik, N. Delsuc, C. Policar, “Intracellular location matters: rationalization of the anti-inflammatory activity of a manganese(ii) superoxide dismutase mimic complex” Chem. Commun. 2020, 56, 7885–7888. [8] M. Zoumpoulaki, G. Schanne, N. Delsuc, H. Preud’homme, E. Quévrain, N. Eskenazi, G. Gazzah, R. Guillot, P. Seksik, J. Vinh, R. Lobinski, C. Policar, “Deciphering the Metal Speciation in Low‐Molecular‐Weight Complexes by IMS‐MS: Application to the Detection of Manganese Superoxide Dismutase Mimics in Cell Lysates” Angew. Chem. Int. Ed. 2022, 61, e202203066. [9] M. Zoumpoulaki, G. Chiappetta, J. Bouvet, N.-R. John, G. Schanne, P. Gehan, S. Diebolt, S. Shakir, E. Quévrain, E. Mathieu, S. Demignot, P. Seksik, N. Delsuc, J. Vinh, C. Policar, “Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic” Angew. Chem. Int. Engl. 2025, e202422644. [10] G. Schanne, A. Vincent, F. Chain, P. Ruffié, C. Carbonne, E. Quévrain, E. Mathieu, A. Balfourier, L. E. Bermudez, P. Langella, S. Thenet, V. Carrière, N. Hammoudi, M. Svreck, S. Demignot, P. Seksik, C. Policar, N. Delsuc, “SOD mimics delivered to the gut using lactic acid bacteria mitigate the colitis symptoms in a mouse model of Inflammatory Bowel Diseases” Free Radical Research 2025, 59, 262–273. [11] D. J. Hare, E. J. New, M. D. de Jonge, G. McColl, “Imaging metals in biology: balancing sensitivity, selectivity and spatial resolution” Chem. Soc. Rev. 2015, 44, 5941–5958. [12] S. Clède, C. Policar, “Metal-Carbonyl Units for Vibrational and Luminescence Imaging: Towards Multimodality” Chem. Eur. J. 2015, 21, 942–958. [13] S. Hostachy, M. Masuda, T. Miki, I. Hamachi, S. Sagan, O. Lequin, K. Medjoubi, A. Somogyi, N. Delsuc, C. Policar, “Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins” Chem. Sci. 2018, 9, 4483–4487. [14] G. Schanne, L. Henry, H. C. Ong, A. Somogyi, K. Medjoubi, N. Delsuc, C. Policar, F. García, H. C. Bertrand, “Rhenium carbonyl complexes bearing methylated triphenylphosphonium cations as antibody-free mitochondria trackers for X-ray fluorescence imaging” Inorg. Chem. Front. 2021, 8, 3905–3915. [15] S. Clède, F. Lambert, C. Sandt, Z. Gueroui, M. Réfrégiers, M.-A. Plamont, P. Dumas, A. Vessières, C. Policar, “A rhenium tris-carbonyl derivative as a single core multimodal probe for imaging (SCoMPI) combining infrared and luminescent properties” Chem. Commun. 2012, 48, 7729. |
| Language | English |
| Intended for | All TUAT members are welcome to join. |
| Organized by | Institue of Global Innovation Research, "LIFE SCIENCE" Sakurai Team |
| Contact | Institute of Global Innovation Research, Institute of Enginnering Prof. Kaori Sakurai e-mail: sakuraik (at) cc.tuat.ac.jp |
| Remarks | ※On-line Seminar by Zoom
＜Biography＞
She has been the principal investigator in more than ten projects funded by a large range of different funding bodies (ANR, Fondation pour la recherche médicale, CNRS, Idex, ministère de la recherche, associations de malades, CNRS, CEFIPRA).
Clotilde Policar has been strongly involved into service to the scientific community. Besides being now the past-president of SBIC (society in bioinorganic chemistry), she was an editorial board member of the Journal of Inorganic Biochemistry, advisory board member for the ACS Inorganic Chemistry and a former deputy science director (DAS) at the Institut de chimie (INC) in CNRS (Centre National de la Recherche Scientifique, France), where she was involved in promoting multidisciplinarity at the level of the CNRS (national-wide service). Since 2020, she is the dean of the sciences education Ecole normale supérieure where she focuses on bridging literature and science, both at the teaching and research level, echoing her own work with theater to popularize science. In 2025, she chaired the International Symposium in Metallomics ISM-10 at ENS-PSL.
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