|Date||2023.6.12 (16：00 - 17：00)|
Multipurpose Lecture Room 1st Fl., Building 2 Fuchu Campus, TUAT
Meeting ID:770 525 2987
|Speaker||Dr. Hisashi Koiwa|
|Affiliation||Texas A&M University (U.S.A.)|
|Title||"Heat stress triggers noncanonical turnover pathway for plasma membrane protein KOR1"
In plants, turnover of plasma membrane (PM) proteins is essential during acclimation to various biotic and abiotic stresses, yet little is known about the mechanisms that detect and replace damaged proteins at the PM. Arabidopsis thaliana KORRIGAN1 (KOR1) is a membrane-bound β1,4-glucanase essential for cellulose biosynthesis. Structurally compromised KOR1, most prominently under heat stress, are diverted from the PM-trans-Golgi network (TGN) cycle and delivered to the tonoplast (TP), which contrasts with the canonical PM protein degradation route via endosomes to vacuoles. Genetic, pharmacological, and imaging analyses revealed that TP targeting process of KOR1 depends on transport machineries in PM and TGN and goes through multivesicular endosome (MVE) and pre-vacuolar compartment (PVC), demonstrating that KOR1 takes the TGN-MVE/PVC-TP route for degradation. Unlike with the canonical vacuolar targeting/degradation substrates, KOR1 transport did not appear to involve ubiquitination, but phosphorylation at the cytoplasmic domain strongly influenced TP targeting. Thus, TP targeting of KOR1 represents a non-canonical pathway for PM protein degradation and highlights the importance of TGN function for sorting decisions on plant PM-protein turnover.
|Intended for||Everyone is welcome to join.|
|Co-Organized by||Institute of Global Innovation Research "FOOD" Fukuhara Team
Excellent Leader Development for Super Smart Society by New Industry Creation and Diversity
|Contact||Institute of Global Innovation Research, Institute of Agriculture
Prof. Toshiyuki Fukuhara
Email: fuku(at) cc.tuat.ac.jp
This seminar will be held both face-to-face and online concurrently.