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Online Seminar【GIR Open Seminar】 Dr. Richard J. Simpson / La Trobe University (Australia)

Date 2021.11.2 (14:00 - Undecided)
Venue Zoom
Speaker / Topic ※On-line Seminar via Zoom and then video streaming through Google Classroom later.
Join the Zoom Meeting
https://tuat-jp.zoom.us/j/85652630474?pwd=cGljY1ByeVd1d0JxUDNjcTVWQm1xdz09#success
Meeting ID: 856 5263 0474
Pass Code: 261866
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◆Dr. Richard J. Simpson (Distinguished professor, Department of Biochemistry and Genetics, La Trobe University, Australia)
 
◆Title: "Extracellular vesicles: their role in cancer and epithelial-mesenchymal transition"

〈Abstract〉
Epithelial-mesenchymal transition (EMT) is an evolutionary conserved process defined by the loss of epithelial characteristics, and acquisition of the mesenchymal phenotype. In addition to its central role in development, EMT is implicated as a cellular process during tumorigenesis which facilitates tumor cell invasion and metastasis. The EMT molecular machinery can be classified into three broad categories: (i)-inducers (or triggers), a group of upstream growth factors and receptors which initiate the EMT program, (ii)-master transcription factors (EMT-TFs) that orchestrate the EMT program, and (iii)-effectors - a group of proteins responsible for altering cellular features such as morphological change, polarity, and conferring invasive properties. In addition to growth factors and receptors, the last decade has witnessed a growing awareness of the contribution of extracellular vesicles (EVs), notably exosomes, to the EMT process.

Using the oncogenic H-Ras-transformed Madin-Darby Canine Kidney (MDCK) cell line model and a combination of mass spectrometry-based protein profiling (Orbitrap and label-free quantitation), my laboratory has previously identified many extracellular effectors of the EMT programme such as soluble proteins (secretome) involved in ECM (extracellular matrix) remodelling and cell-cell contact. Additionally, we found that H-Ras-induced EMT of MDCK cells resulted in extensive reprogramming of the protein repertoire of exosomes in favour of selected trafficking of cargo proteins known to promote metastatic niche formation, and transcriptional/ splicing factors known to instigate EMT. My laboratory has now extended our studies of the role of EVs in the EMT programme to include a second major class of EVs – microparticles (MPs) - also referred to as shed microvesicles and ectosomes. In contrast to exosomal cargos, tissue transglutaminase-2 (TGM2), RNA binding proteins and mitochondrial proteins selectively traffic to MPs following H-Ras-induced EMT. In this seminar I will also discuss functional differences between exosomes and MPs in the context of EMT and cancer biology.
Language English
Intended for Everyone is welcome to join.
Co-Organized by Institute of Global Innovation Research ”Life Science” Ikebukuro Team
Excellent Leader Development for Super Smart Society by New Industry Creation and Diversity
Contact Institute of Global Innovation Research, Institute of Agriculture
Professor Hiroko Tabunoki
e-mail: h_tabuno(at)cc.tuat.ac.jp

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