| Date | 2020.12.8 (18：00 - 20：00) |
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| Venue | |
| Title | Google classroom is now available. 1. Go to https://classroom.google.com and sign in. 2. At the upper right, click 「+」 and click 「join」. 3. Enter the class code 27aa3k7. ----------------------------------------------------------------------------------------------- ※On-line Seminar via Zoom and Google Classroom. Registration is required for the Zoom meeting. Please contact Prof. Kajita (e-mail: kajita@cc.tuat.ac.jp). ------------------------------------------------------------------------------------------------ Associate Prof. Edouard Pesquet Department of Ecology, Environment and Plant Sciences Stockholm University, Sweden "Japanese-Swedish collaboration (TUAT/SU) to understand the cell biology of lignification" -Abstract Lignins are phenolic heteropolymers which accumulate in cell walls to confer specialized functions to specific cell types such as mechanical resistance, sap conduction or apoplast impermeabilisation. Specific lignins are thus deposited in these different cell types and vary in amounts, composition and localization in the different layers of their cell walls, but also dynamically change to adapt to developmental and environmental constrains [1]. This cellular complexity has represented an important unknown, which hindered the complete understanding of plant lignification and the optimal use of plant biomass for biorefinery processes. A collaboration initiated in 2015 between the research groups of Prof. Shinya Kajita (TUAT, Tokyo, Japan) and Assoc.Prof. Edouard Pesquet (SU, Stockholm, Sweden) was established to investigate the cellular regulation of lignification in both herbaceous and woody plants. To do so, the joint research effort focused on the optimisation of in situ quantification techniques on whole plant biopsies to directly monitor lignin properties (amount and composition) at the sub-cellular and cellular levels. Two wide-field microspectroscopy methods were optimized, using "RGB-absorbance" and Raman spectroscopy, and validated on sets of synthetic monomers and polymers as well as many genetically engineered plants with modified lignins [2-4]. These optimized methods revealed that the lignification of each cell types resulted from a combination of specific genetic processes, different levels of cellular cooperation and cell wall specific lignin accumulation capacities, which varied during plant development not only to control lignin amount and composition but also residue position in the lignin polymers [2-4]. In the present seminar, these different methods will be presented as well as novel unpublished data on the use of these methods to elucidate the regulation processes controlling the lignification of each specific cell types. -References [1] Pesquet et al. (2019) Current Opinion in Plant Biotechnology - https://doi.org/10.1016/j.copbio.2019.02.001 [2] Blaschek et al., (2020a) Frontiers in Plant Sciences - https://doi.org/10.3389/fpls.2020.00109 [3] Blaschek et al., (2020b) ACS Sustainable Chemistry and Engineering - https://doi.org/10.1021/acssuschemeng.0c00194 [4] Yamamoto et al., (2020) ChemSusChem - https://doi.org/10.1002/cssc.202001242 |
| Language | English |
| Intended for | Registration is required. |
| Co-Organized by | Institute of Global Innovation Research, "Food" group Excellent Leader Development for Super Smart Society by New Industry Creation and Diversity |
| Contact | Institute of Global Innovation Research, Institute of Agriculture Professor Shinya Kajita e-mail: kajita(at)cc.tuat.ac.jp |
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