|Affiliation||Institute of Engineering|
【LIFE SCIENCE】 Kuroda Unit
We are developing novel bifunctional fluorescent and chemiluminescent probes to detect and image bacteria in vivo. We designed several enzyme-substrate based chemiluminescent probes, whose chemiluminesce is triggered by active enzymes for real-time in vivo imaging at cellular level. For examples, the β-lactam chemiluminescent probes can be applied to detect the antibiotic resistance level and distribution during bacterial infections. Meanwhile, we also designed novel multi-functional florescent probes for bacterial strain identification. These probes are meant to selectively label specific species of bacteria for in vivo imagining applications, such as host-pathogen, host-microbiota and pathogen-microbiota interactions in a real-time manner.
In addition, we are developing selective chemical labeling methods to explore secondary metabolites produced by complex biological systems. Our goal is to discover novel secondary metabolites, which are difficult to be obtain using conventional approaches. We first focus our efforts on designing chemical labeling methods on ubiquitous active aromatic and nonpolar motifs in NRP (Non Ribosomal Peptides). We will apply these chemical labeling methods, combined with mass spectrometry and bioinfomatics, to identify unknown NRPs in complex biological systems, such as the soil or the human microbiota.
|Affiliation||National Taiwan University (Taiwan)|
|Division / Department||Department of Chemistry|
|Affiliation||University of Chittagong (Bangladesh)|
|Division / Department||Dept. of Biochemistry & Molecular Biology|